TerSynDB

Plant Name: A4FVP2
Terpene synthases genes
Entry A4FVP2
Entry NameTPS03_ARATH
Sequence Statusreviewed
Protein NameTricyclene synthase, chloroplastic (EC 4.2.3.105) ((E)-beta-ocimene synthase) (EC 4.2.3.106) ((E,E)-alpha-farnesene synthase) (EC 4.2.3.46) (Terpenoid synthase 3) (AtTPS03)
Sequence Length565
Gene NameTPS03 At4g16740 dl4395w FCAALL.18
OrganismArabidopsis thaliana (Mouse-ear cress)
EC Number4.2.3.105; 4.2.3.106; 4.2.3.46
Protein FunctionFUNCTION: Predominantly involved in sesquiterpenes (C15) biosynthesis. Using FPP as substrate, the major product is (E,E)-alpha-farnesene with minor amounts of (Z,E)-alpha-farnesene and (E,E)-beta-farnesene. Using GPP as substrate, could also be able in vitro to synthesize monoterpene (C10) with (E)-beta-ocimene as the major product and with (Z)-beta-ocimene and myrcene as minor products. {ECO:0000269|PubMed:12624761, ECO:0000269|PubMed:20463089}.
PathwayPATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
Protein Active Site
Reference9461215; 10617198; 11910074; 12624761; 11710601; 12207221; 12566586; 12777052; 16297850;
DomainDOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+).
MotifMOTIF 320 324 DDXXD motif.
Protein FamilyTerpene synthase family, Tpsb subfamily
Ensembl IDAT4G16740.1 [A4FVP2-1];AT4G16740.2 [A4FVP2-2];
Biocyc IDPF01397;PF03936;
Protein ExistenceEvidence at transcript level
Subunit Structure
Gene Ontology Biological Process response to herbivore [GO:0080027]; response to insect [GO:0009625]; response to jasmonic acid [GO:0009753]; response to wounding [GO:0009611]; sesquiterpenoid biosynthetic process [GO:0016106]
Gene Ontology Cellular Componentchloroplast stroma [GO:0009570]; cytosol [GO:0005829]
Gene Ontologychloroplast stroma [GO:0009570]; cytosol [GO:0005829]; (E)-beta-ocimene synthase activity [GO:0034768]; alpha-farnesene synthase activity [GO:0052578]; magnesium ion binding [GO:0000287]; myrcene synthase activity [GO:0050551]; terpene synthase activity [GO:0010333]; tricyclene synthase activity [GO:0102701]; response to herbivore [GO:0080027]; response to insect [GO:0009625]; response to jasmonic acid [GO:0009753]; response to wounding [GO:0009611]; sesquiterpenoid biosynthetic process [GO:0016106]
Gene Ontology Molecular Function (E)-beta-ocimene synthase activity [GO:0034768]; alpha-farnesene synthase activity [GO:0052578]; magnesium ion binding [GO:0000287]; myrcene synthase activity [GO:0050551]; terpene synthase activity [GO:0010333]; tricyclene synthase activity [GO:0102701]
GO IDGO:0000287; GO:0005829; GO:0009570; GO:0009611; GO:0009625; GO:0009753; GO:0010333; GO:0016106; GO:0034768; GO:0050551; GO:0052578
String Database3702.AT4G16740.1;
PDB
RefSeqNP_001031651.1 [A4FVP2-2];NP_567511.3 [A4FVP2-1];
KEGGath:AT4G16740;
InterProIPR008949;IPR034741;IPR001906;IPR036965;IPR005630;IPR008930;
Protein SequenceMPKRQAQRRFTRKTDSKTPSQPLVSRRSANYQPSLWQHEYLLSLGNTYVKEDNVERVTLLKQEVSKMLNETEGLLEQLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQHGFSIAQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTKRLRKFVEVNKNETKSYTLRRMVIHALEMPYHRRVGRLEARWYIEVYGERHDMNPILLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDVNRLEELPNYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFEEYMQNGWISSSVPTILLHLFCLLSDQTLDILGSYNHSVVRSSATILRLANDLATSSEELARGDTMKSVQCHMHETGASEAESRAYIQGIIGVAWDDLNMEKKSCRLHQGFLEAAANLGRVAQCVYQYGDGHGCPDKAKTVNHVRSLLVHPLPLN
CSIR-CIMAP