TerSynDB

Plant Name: G9MAN7
Terpene synthases genes
Entry G9MAN7
Entry NameTPS4_SELML
Sequence Statusreviewed
Protein NameBifunctional diterpene synthase, chloroplastic (Copalyl diphosphate synthase) (EC 5.5.1.12) (Miltiradiene synthase) (SmMDS) (EC 4.2.3.131) (Terpene synthase 4) (SmTPS4)
Sequence Length867
Gene NameMDS SELMODRAFT_450918
OrganismSelaginella moellendorffii (Spikemoss)
EC Number5.5.1.12; 4.2.3.131
Protein FunctionFUNCTION: Bifunctional diterpene cyclase that catalyzes the successive two-step type-B (protonation-initiated cyclization) and type-A (ionization-initiated cyclization) reactions of geranylgeranyl diphosphate (GGDP) producing successively (+)-copalyl diphosphate and miltiradiene. {ECO:0000269|PubMed:22027823}.
PathwayPATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
Protein Active Site
Reference22027823; 21551031; 22908266
DomainDOMAIN: The Asp-Xaa-Asp-Asp (DXDD) and Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motifs are important for the catalytic activities, presumably through binding to Mg(2+). {ECO:0000250}.
MotifMOTIF 389 392 DXDD motif.; MOTIF 611 615 DDXXD motif.
Protein FamilyTerpene synthase family
Ensembl ID
Biocyc IDPF01397;PF03936;
Protein ExistenceEvidence at protein level
Subunit Structure
Gene Ontology Biological Process 5alpha,9alpha,10beta-labda-8(20),13-dien-15-yl diphosphate biosynthetic process [GO:1901949]; 5alpha,9alpha,10beta-labda-8(20),13-dien-15-yl diphosphate catabolic process [GO:1901948]; miltiradiene biosynthetic process [GO:1901946]
Gene Ontology Cellular Componentchloroplast [GO:0009507]
Gene Ontologychloroplast [GO:0009507]; copalyl diphosphate synthase activity [GO:0050559]; magnesium ion binding [GO:0000287]; multiradiene synthase activity [GO:0102353]; terpene synthase activity [GO:0010333]; 5alpha,9alpha,10beta-labda-8(20),13-dien-15-yl diphosphate biosynthetic process [GO:1901949]; 5alpha,9alpha,10beta-labda-8(20),13-dien-15-yl diphosphate catabolic process [GO:1901948]; miltiradiene biosynthetic process [GO:1901946]
Gene Ontology Molecular Function copalyl diphosphate synthase activity [GO:0050559]; magnesium ion binding [GO:0000287]; multiradiene synthase activity [GO:0102353]; terpene synthase activity [GO:0010333]
GO IDGO:0000287; GO:0009507; GO:0010333; GO:0050559; GO:0102353; GO:1901946; GO:1901948; GO:1901949
String Database
PDB
RefSeqXP_002967117.1;
KEGGsmo:SELMODRAFT_450918;
InterProIPR008949;IPR001906;IPR036965;IPR005630;IPR008930;
Protein SequenceMAKVLFSSFQQTGISGSLKSGQLSGVFINGTNLKSNAHAKRFRKNSTSSITIRCCASNSPTLENTKLAGAPEKRQKKKQLPYQGILHVPGDRVEELDTRETSLLVAEVKGWLMKLASGKGEISPSAYDTAWVARIASESDSSLPEFPEALEWIINSQLPDGSWGDDRHLQLYDRVLSTLSCLVTLKTWDIGHNSIAQGTKFLRENMIKLKQDDGDLLSGFEVTFPMMLHEAKQLGLDIPYETEFTRLLEISTKKKLAKIPLDKLHSAPTTLLYSLEGLQDLEIDWQKILKLQSKDGSFLSSPSSTACVYLKTKDRKSLQYLQNAMEDQNYAVPCHYPIDLFESLWVVDTIERLGIDVFFRDEIKAVLDYVYSFWTNEGIGWGSTCLVNDIDDTAMAFRILRMHGYNVSPDAFNQFWLPGDKFCCFVGELSHGVSEMLNLHRASQVDFPNEAILTKTFKYSHDYLLNVDSAHMDKWATKKNLMGEVAFELANPFHDCLPRIYNNAYIKHYGMDDLWIAKTIYRLPLVNNKVFLELANRYAQQCQLYQPAELTKLVNWWHSSRFEDIPSTRLTANIDMLPYIYYVICATFHEQEFAQLRVFFSKACCLNTLFDDLMDCATSIEELDRLQNVIERWDISLSHELPLEYRIPFQEFYNTVLVMTEAASKIHKNLSPEFICKYLSGIYTKLIKSEIADARWKIEGYIPSFEEYMENAEVSISTWVHVLMSILFCGEPLTEEILNTIYDSRPLKLDRIICRLCNDIQTYKIEMKLGQPTQGVSCYMKEHPGATEEDALVYLQSLLEKTKRELNESYFITHENDLPKNIKRFNFEMVRMMLITYNETRQVDLFRNPDNELKDMIKFCLETYRTL
CSIR-CIMAP