| Entry | Q40577 |
Entry Name | 5EAS_TOBAC |
| Sequence Status | reviewed |
| Protein Name | 5-epi-aristolochene synthase (EAS) (EC 4.2.3.61) |
| Sequence Length | 548 |
| Gene Name | EAS3; EAS4 |
| Organism | Nicotiana tabacum (Common tobacco) |
| EC Number | 4.2.3.61 |
Protein Function | FUNCTION: Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene. |
Pathway | PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis. |
Protein Active Site | |
Reference | 1438319; 9295271 |
Domain | DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+). |
Motif | MOTIF 301 305 DDXXD motif. |
Protein Family | Terpene synthase family |
Ensembl ID | |
Biocyc ID | PF01397;PF03936; |
Protein Existence | Evidence at protein level |
Subunit Structure | SUBUNIT: Monomer. {ECO:0000269|PubMed:9295271}. |
Gene Ontology Biological Process | terpenoid biosynthetic process [GO:0016114] |
Gene Ontology Cellular Component | cytoplasm [GO:0005737] |
Gene Ontology | cytoplasm [GO:0005737]; 5-epi-aristolochene synthase activity [GO:0102698]; magnesium ion binding [GO:0000287]; terpene synthase activity [GO:0010333]; terpenoid biosynthetic process [GO:0016114] |
Gene Ontology Molecular Function | 5-epi-aristolochene synthase activity [GO:0102698]; magnesium ion binding [GO:0000287]; terpene synthase activity [GO:0010333] |
GO ID | GO:0000287; GO:0005737; GO:0010333; GO:0016114; GO:0102698 |
String Database | |
PDB | 1HX9;1HXA;1HXC;1HXG;3LZ9;3M00;3M01;3M02;4DI5;4RNQ;5DHI;5DHK;5EAS;5EAT;5EAU;5IK0;5IK6;5IK9;5IKA;5IKH;5IL3;5IL8;5ILD;5ILH;5ILI;5ILJ;5ILK;5ILY;5ILZ;5IM1; |
RefSeq | |
KEGG | ag:AAA19216; |
InterPro | IPR008949;IPR034741;IPR001906;IPR036965;IPR005630;IPR008930; |
Protein Sequence | MASAAVANYEEEIVRPVADFSPSLWGDQFLSFSIKNQVAEKYAKEIEALKEQTRNMLLATGMKLADTLNLIDTIERLGISYHFEKEIDDILDQIYNQNSNCNDLCTSALQFRLLRQHGFNISPEIFSKFQDENGKFKESLASDVLGLLNLYEASHVRTHADDILEDALAFSTIHLESAAPHLKSPLREQVTHALEQCLHKGVPRVETRFFISSIYDKEQSKNNVLLRFAKLDFNLLQMLHKQELAQVSRWWKDLDFVTTLPYARDRVVECYFWALGVYFEPQYSQARVMLVKTISMISIVDDTFDAYGTVKELEAYTDAIQRWDINEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIVCHAIERMKEVVRNYNVESTWFIEGYTPPVSEYLSNALATTTYYYLATTSYLGMKSATEQDFEWLSKNPKILEASVIICRVIDDTATYEVEKSRGQIATGIECCMRDYGISTKEAMAKFQNMAETAWKDINEGLLRPTPVSTEFLTPILNLARIVEVTYIHNLDGYTHPEKVLKPHIINLLVDSIKI |
